When are blood samples collected during the five-hour diagnostic test with Synacthen Depot (tetracosactide acetate) 1 mg/ml?

In adults undergoing the five-hour Synacthen Depot (tetracosactide acetate) 1 mg/ml diagnostic test, blood samples for plasma cortisol measurement are collected before and exactly 30 minutes, 1, 2, 3, 4 and 5 hours after an intramuscular injection of 1 mg.