Which diagnostic tests are recommended for confirming a diagnosis of rectal cancer in primary care?

Guideline-aligned answer with reasoning, red flags and references. Clinically reviewed by Dr Kola Tytler MBBS CertHE MBA MRCGP.

Posted: 22 August 2025Updated: 22 August 2025 Guideline-Aligned (High Confidence) Clinically Reviewed
Dr Kola Tytler MBBS CertHE MBA MRCGPClinical Lead • iatroX

In primary care, the recommended diagnostic approach to confirm a diagnosis of rectal cancer begins with a thorough clinical assessment including a digital rectal examination (DRE) to detect any rectal mass, which if found, warrants urgent referral via a suspected cancer pathway . Following this, quantitative faecal immunochemical testing (FIT) is advised for patients presenting with symptoms suggestive of colorectal cancer, such as iron-deficiency anaemia or non-iron-deficiency anaemia in those aged 60 and over, to guide the need for further investigation . FIT serves as a non-invasive test to stratify risk and prioritize patients for colonoscopy or other definitive diagnostic procedures . If FIT is positive or if there is a palpable rectal mass on DRE, an urgent referral for colonoscopy or flexible sigmoidoscopy is indicated to visually confirm the presence of rectal cancer and obtain biopsy samples for histological diagnosis .

While DRE remains a cornerstone in initial assessment, its sensitivity and specificity can vary; meta-analyses in related cancer screening contexts (e.g., prostate cancer) highlight that DRE alone is insufficient for definitive diagnosis but valuable as part of the clinical evaluation . Therefore, DRE should be combined with FIT and prompt referral for endoscopic evaluation to confirm rectal cancer diagnosis . Imaging such as CT scanning is generally reserved for staging after diagnosis rather than initial confirmation in primary care .

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