What are the key diagnostic criteria for confirming a Clostridium difficile infection in a patient presenting with diarrhea?

Guideline-aligned answer with reasoning, red flags and references. Clinically reviewed by Dr Kola Tytler MBBS CertHE MBA MRCGP.

Posted: 16 August 2025Updated: 16 August 2025 Guideline-Aligned (High Confidence) Clinically Reviewed
Dr Kola Tytler MBBS CertHE MBA MRCGPClinical Lead • iatroX

Confirming a Clostridioides difficile infection (CDI) in a patient presenting with diarrhoea primarily relies on a positive C. difficile toxin test . However, this test result must be interpreted in conjunction with strong clinical suspicion .

It is important to note that C. difficile may be an incidental finding in the stools of people with diarrhoea caused by other factors, and indiscriminate testing increases the likelihood of a false-positive result . Colonisation with C. difficile is common, and individuals who are colonised may be asymptomatic .

If a patient has a negative C. difficile toxin test result but there is a strong clinical suspicion of CDI, or if there is doubt, it is advisable to consider seeking specialist advice and potentially retesting . North American guidelines also recommend considering repeat testing in symptomatic individuals with a negative result if there is a high clinical suspicion of CDI . This approach is consistent with European guidelines and Public Health England advice, which acknowledge that a single negative result can occur .

Educational content only. Always verify information and use clinical judgement.